Automated tilt series alignment and tomographic reconstruction in IMOD

A marker-free alignment of tilt series images for tomographic reconstruction

3D reconstruction of biological structures: automated procedures for alignment and reconstruction of multiple tilt series in electron tomography

Transmission electron microscopy allows the collection of multiple views of specimens and their computerized three-dimensional reconstruction and analysis with electron tomography. Here we describe development of methods for automated multi-tilt data acquisition, tilt-series processing, and alignment which allow assembly of electron tomographic data from a greater number of tilt series, yieldin...

Tomographic reconstruction of combined tilt- and focal series in scanning transmission electron microscopy

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Automated Procedures for the Alignment and Reconstruction of Multiple Tilt Electron Microscopic Tomography Data

Transmission electron microscopy, combined with tomographic techniques, has been used for decades to generate 3D representations of biological structures, with resolutions down to the nanometer range [1,2]. Here, we describe a method, extending the Transform-Based Reconstruction (TxBR) method [3,4] whereby large field electron tomography (ET) can be optimized both in terms of data acquisition a...

Partially Observed Tomographic Reconstruction Alignment Using Matrix Norm Minimization

In this project, we propose a new algorithm that recovers the rigid motion parameters and the underlying density function from a set of noisy measurements of three dimensional (3D) density functions. We measure the Radon transform of each 3D function which are randomly rotated and translated version of a target function that we try to recover. Radon transform is a set of parallel line integrals...